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Journal / Immunology and Infection / Generazione di vettori ricombinanti Herpesvirus…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Generazione di vettori ricombinanti Herpesvirus aviaria con CRISPR/Cas9 Gene Editing
Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis
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Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis
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Generazione di vettori ricombinanti Herpesvirus aviaria con CRISPR/Cas9 Gene Editing

Article DOI: 10.3791/58193-v • 12:21 min • January 7th, 2019
January 7th, 2019 •
1,2, 1, 1, 1, 1, 1, 2, 1, 1
1The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, 2Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

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Herpesvirus dei tacchini (HVT) è ampiamente usato come una piattaforma di vettore per la generazione di vaccini ricombinanti contro un certo numero di malattie aviarie. Questo articolo viene descritto un approccio semplice e rapido per la generazione di vaccini ricombinanti HVT-vectored utilizzando un sistema di Cre-Lox e integrato NHEJ-CRISPR/Cas9.

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Immunologia e infezione numero 143 CRISPR/Cas9 NHEJ Cre-LoxP HVT vaccino ricombinante malattie aviarie
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