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Journal / Biochemistry / 用石英谐振器研究磷脂结合蛋白附件 a2…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
用石英谐振器研究磷脂结合蛋白附件 a2 与固体支持的脂质层结合动力学的耗散微重法
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
Journal (Biochemistry)
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
Journal (Biochemistry)
Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
 
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用石英谐振器研究磷脂结合蛋白附件 a2 与固体支持的脂质层结合动力学的耗散微重法

Article DOI: 10.3791/58224-v • 07:11 min • November 1st, 2018
November 1st, 2018 •
*1,3, *1,3, 1,3, 1,3, 2,3, 1,3, 1,3
1Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation, University of Münster, 2Institute of Biochemistry, University of Münster, 3Cluster of Excellence 'Cells in Motion', University of Münster
* These authors contributed equally

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在这里, 我们提出了一个实验协议, 可用于确定结合亲和力和模式的相互作用, 无标签磷脂结合蛋白 a2 与固定化固体支持的双层 (slb) 同时测量蛋白质附件 a2 的质量吸收和粘弹性特性。

Tags

生物化学 第141期 微平衡 固体支持的双层 磷脂微域 胆固醇 烯烃 合作性 结合动力学 粘弹性特性
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