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Journal / Immunology and Infection / Cas9 Ribonucleoproteins का उपयोग करके नॉक-आउट…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Cas9 Ribonucleoproteins का उपयोग करके नॉक-आउट प्राथमिक और विस्तारित मानव NK कोशिकाओं का निर्माण
Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model
Journal (Immunology and Infection)
Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model
Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes
Journal (Immunology and Infection)
Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes
 
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Cas9 Ribonucleoproteins का उपयोग करके नॉक-आउट प्राथमिक और विस्तारित मानव NK कोशिकाओं का निर्माण

Article DOI: 10.3791/58237-v • 07:26 min • June 14th, 2018
June 14th, 2018 •
1, 2, 1, 3, 1, 1, 1, 1, 1, 1, 1, 3, 1
1Center for Childhood Cancer and Blood Disease, Nationwide Children's Hospital, 2Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine, University of Oxford, 3Center for Clinical and Translational Research, Nationwide Children's Hospital Research Institute

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यहां, हम आनुवंशिक रूप से संशोधित करने के लिए एक प्रोटोकॉल वर्तमान प्राथमिक या विस्तारित मानव प्राकृतिक हत्यारा (NK) कोशिकाओं का उपयोग Cas9 Ribonucleoproteins (Cas9/RNPs) । इस प्रोटोकॉल का उपयोग करके, हम मानव NK वृद्धि कारक-बी रिसेप्टर 2 (TGFBR2) और hypoxanthine phosphoribosyltransferase 1 (HPRT1) को बदलने के लिए कमी कोशिकाओं उत्पन्न.

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इम्यूनोलॉजी और संक्रमण अंक १३६ मानव प्राथमिक NK कोशिकाओं मानव विस्तारित NK कोशिकाओं CRISPR/Cas9 Cas9/RNPs gRNAs TGFBR2 TGFβ प्रतिरोधी NK कोशिकाओं
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