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JoVE Journal Genetics
Producerar genen borttagningar i Escherichia coli av P1 transduktion med punktskattepliktiga antibiotikaresistens kassetter
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Producerar genen borttagningar i Escherichia coli av P1 transduktion med punktskattepliktiga antibiotikaresistens kassetter

Article DOI: 10.3791/58267-v • 08:13 min • September 1st, 2018
September 1st, 2018 •
1, 1, 1
1Evolution and Genetics, Department of Biosciences, University of Oslo

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Här presenterar vi ett protokoll för användning av befintlig antibiotika resistens-kassett radering konstruktioner som underlag för att göra strykningen mutanter i andra E. coli -stammar. Sådan radering mutationer kan mobiliseras och infogas i det motsvarande locus av en mottagare stam med P1 bacteriophage transduktion.

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Genetik fråga 139 antibiotikaresistens kassett radering mutagenes FLP recombinase P1 transduktion flyttning och församling modul trimeric autotransporter adhesin
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