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Cancer Research

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JoVE Journal Cancer Research
En kromatin Immunoprecipitation analyse til at identificere nye NFAT2 Target gener i kronisk lymfatisk leukæmi
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En kromatin Immunoprecipitation analyse til at identificere nye NFAT2 Target gener i kronisk lymfatisk leukæmi

Article DOI: 10.3791/58270-v • 09:52 min • December 4th, 2018
December 4th, 2018 •
1, 1, 1, 1, 2, 1, 1, 1, 1, 1
1Dept. of Hematology, Oncology and Immunology, University of Tübingen, 2Dept. of Endocrinology, Diabetology, Clinical Pathology and Metabolism, University of Tübingen

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Kronisk lymfatisk leukæmi (CLL) er den mest almindelige leukæmi i den vestlige verden. NFAT transkriptionsfaktorer er vigtige regulatorer af udvikling og aktivering i talrige celletyper. Vi præsenterer her, en protokol til brug af kromatin immunoprecipitation (ChIP) i humane CLL celler til at identificere nye mål gener af NFAT2.

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Kræftforskning sag 142 CLL NFAT transkriptionsfaktor target gen initiativtageren kromatin immunoprecipitation
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