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JoVE Journal Biochemistry
Endre Baculovirus uttrykk vektorer å produsere utskilles anlegget proteiner i insekt celler
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Endre Baculovirus uttrykk vektorer å produsere utskilles anlegget proteiner i insekt celler

Article DOI: 10.3791/58283-v • 09:14 min • August 20th, 2018
August 20th, 2018 •
1, 1, 1
1Department of Molecular and Structural Biochemistry, College of Agriculture and Life Sciences, North Carolina State University

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Her presenterer vi protokollene for utnytte insekt celler og baculovirus protein uttrykk systemet å produsere store mengder anlegget utskilles proteiner for protein krystallisering. En baculovirus uttrykk vektor er endret med GP67 eller insekt hemolin signal peptid plante protein sekresjon uttrykk i insekt celler.

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Biokjemi problemet 138 insekt celler baculovirus uttrykk vektor sekretoriske protein signal peptid GP67 hemolin bacmid hva cellekultur glykosylering protein uttrykk protein krystallisering
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