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Journal / Biochemistry / Ændre Baculovirus udtryk vektorer til at…
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Biochemistry

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JoVE Journal Biochemistry
Ændre Baculovirus udtryk vektorer til at producere udskilles vegetabilske proteiner i insekt celler
Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
Journal (Biochemistry)
Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
Journal (Biochemistry)
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
 
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Ændre Baculovirus udtryk vektorer til at producere udskilles vegetabilske proteiner i insekt celler

Article DOI: 10.3791/58283-v • 09:14 min • August 20th, 2018
August 20th, 2018 •
1, 1, 1
1Department of Molecular and Structural Biochemistry, College of Agriculture and Life Sciences, North Carolina State University

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Vi præsenterer her, protokoller for at udnytte den insekt celle og baculovirus protein udtryk system til at producere store mængder af planten udskilles protein til protein krystallisering. Et baculovirus udtryk vektor er blevet ændret med enten GP67 eller insekt hemolin signal peptid for plante protein sekretion udtryk i insekt celler.

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Biokemi spørgsmålet 138 insekt celle baculovirus udtryk vektor sekretoriske proteiner signal peptid GP67 hemolin bacmid Transfektion cellekultur glykosylering protein udtryk protein krystallisering
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