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Immunology and Infection

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JoVE Journal Immunology and Infection
Antigena liposomer för generering av sjukdom-specifika antikroppar
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Antigena liposomer för generering av sjukdom-specifika antikroppar

Article DOI: 10.3791/58285-v • 10:31 min • October 25th, 2018
October 25th, 2018 •
*1, *2,3, *3,4, 5, 6, 3,4, 5,7
1Janssen R&D, 2Department of Microbiology and Immunology, University of North Carolina, 3UNC Food Allergy Initiative, University of North Carolina, 4Department of Pediatrics, University of North Carolina, 5Department of Chemistry, University of Alberta, 6Department of Molecular Medicine, Scripps Research Institute, 7Department of Medical Microbiology and Immunology, University of Alberta
* These authors contributed equally

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Beskrivs är utarbetandet av antigena liposomalt nanopartiklar och deras användning i stimulerande B-cells aktivering in vitro- och in-vivo. Konsekvent och robust antikroppssvar ledde till utvecklingen av en ny jordnötsallergi modell. Protokollet för att generera antigena liposomer kan utvidgas till olika antigener och immunisering modeller.

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Immunologi och infektion fråga 140 nanopartiklar liposomer anafylaxi jordnöt allergi kalcium Flux Flow flödescytometri specifik Antigen Ara h 2 födoämnesallergi IgE
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