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JoVE Journal Developmental Biology
Analyse van epididymaire eiwitsynthese en afscheiding
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Analyse van epididymaire eiwitsynthese en afscheiding

Article DOI: 10.3791/58308-v • 10:23 min • August 25th, 2018
August 25th, 2018 •
1,2, 3, 1,2, 2,4, 1,2
1Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, 2Hunter Medical Research Institute, 3Department of Physiology, Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, 4School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle

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Wij rapporteren hier, de lokalisatie van de immunofluorescentietest voor dynamin ter illustratie van de protocollen voor de detectie van eiwitten in paraffine-ingebedde muis epididymaire secties en die van een vereeuwigd epididymaire cellijn (mECap18). Ook beschrijven we de protocollen voor de isolatie van proteïnen van de secretoire van zowel epididymaire vloeistof en geconditioneerde cel media.

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Ontwikkelingsbiologie kwestie 138 Dynamin bijbal epididymosome exosome immunofluorescentie eiwit secretie sperma sperma rijping
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