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Isolasjon hepatocytes hovedknappen på musen for begynnende Protein syntese analyse av ikke-radioaktivt L-azidohomoalanine merking metoden
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Isolasjon hepatocytes hovedknappen på musen for begynnende Protein syntese analyse av ikke-radioaktivt L-azidohomoalanine merking metoden

Article DOI: 10.3791/58323-v • 08:04 min • October 23rd, 2018
October 23rd, 2018 •
*1,2, *2, 2, 2, 2, 2, 2,3,4
1Department of Pharmacology and Systems Physiology, College of Medicine, University of Cincinnati, 2Division of Endocrinology, Cincinnati Children's Hospital Medical Center, 3Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 4Department of Pediatrics, College of Medicine, University of Cincinnati
* These authors contributed equally

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Her presenterer vi en protokoll for isolasjon hepatocytes sunn og funksjonelle hovedknappen på musen. Instruksjoner for å oppdage hepatic begynnende proteinsyntese av ikke-radioaktivt merking underlaget ble gitt til å forstå mekanismene bak proteinsyntese i sammenheng med energi-metabolisme homeostase i leveren.

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Medisin problemet 140 hovedknappen på musen hepatocytter begynnende proteinsyntese AMPK signalnettverk rotenon L-Azidohomoalanine ikke radioaktiv metode
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