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JoVE Journal
Biology

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激光辅助慢病毒基因传递到小鼠受精卵
 
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激光辅助慢病毒基因传递到小鼠受精卵

Article DOI: 10.3791/58327-v 06:03 min November 1st, 2018
November 1st, 2018

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

小鼠受精卵和早期胚胎受透明带保护, 透明带是一种糖蛋白基质, 形成了对抗基因传递的屏障。本文介绍了一种用激光射孔带带的协议, 用慢病毒载体传递胚胎细胞, 并创造转基因小鼠。

Tags

生物学 第141期 转创 xyclone 激光 慢病毒 小鼠受精卵 小鼠胚胎 基因传递 转导
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