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Laser-assistita lentivirali Gene consegna al Mouse fecondate le uova
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Laser-assistita lentivirali Gene consegna al Mouse fecondate le uova

Article DOI: 10.3791/58327-v • 06:03 min • November 1st, 2018
November 1st, 2018 •
*1, *2, 1, 1, 1, 1, 1, 1, 3, 4, 1
1Neurobiology Laboratory, National Institute of Environmental Health Sciences, 2Comparative Medicine Branch, National Institute of Environmental Health Sciences, 3Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, 4Signal Transduction Laboratory, National Institute of Environmental Health Sciences
* These authors contributed equally

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Mouse di uova fecondate ed embrioni in fase precoce sono protetti dalla zona pellucida, una matrice di glicoproteina che forma una barriera contro la consegna del gene. Questo articolo descrive un protocollo per la zona di perforazione con un laser per trasdurre cellule embrionali con vettori lentivirali e creare topi transgenici.

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Biologia problema 141 transgenesi XYClone laser lentivirus uova fecondata di mouse embrioni di topo consegna del gene trasduzione
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