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Journal / Developmental Biology / C. elegans Germline timidin Analog EdU ile hücre…
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JoVE Journal Developmental Biology
C. elegans Germline timidin Analog EdU ile hücre döngüsü analizi
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C. elegans Germline timidin Analog EdU ile hücre döngüsü analizi

Article DOI: 10.3791/58339-v • 06:58 min • October 22nd, 2018
October 22nd, 2018 •
1,2, 2, 1, 2
1Department of Developmental Biology, Washington University School of Medicine, St. Louis, Missouri, 2Department of Genetics, Washington University School of Medicine, St. Louis, Missouri

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Bir görüntüleme tabanlı yöntemi anlatılan S fazlı tanımlamak ve timidin kullanarak C. elegans hermafrodit germline hücre döngüsü dinamiklerini analiz etmek için kullanılabilir analog EdU. Bu yöntem yok transgenes gerektirir ve immünfloresan boyama ile uyumludur.

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Gelişim biyolojisi sayı: 140 C. elegans EdU S-faz hücre döngüsü Germline segregasyonun S fazlı M-faz G2 fazlı
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