April 2017- This Month in JoVE: Honeybees and Pesticides, Hydrogel Membranes for Water Purification, Language and Cognition in Infants, and Live Imaging of Developing Arabidopsis Flowers

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    Summary

    Date Published: 4/04/2017, Issue 122

    Cite this Article

    Chamberlain, N., Kolski-Andreaco, A. April 2017- This Month in JoVE: Honeybees and Pesticides, Hydrogel Membranes for Water Purification, Language and Cognition in Infants, and Live Imaging of Developing Arabidopsis Flowers. J. Vis. Exp. (122), e5834, (2017).

    Abstract

    Here's a look at what's coming up in the April 2017 issue of JoVE: The World's Premier Video Journal

    The plight of the honeybee holds our first spotlight this month. Pesticides brought back to colonies by workers foraging on contaminated flowers can be spread throughout honeybee colonies, severely impacting health of larvae and adults, and potentially causing colony collapse. In JoVE Environment, our Authors describe feeding methods in which an insect growth regulator and commonly used pesticide - PPN - is fed both to individual honeybees and an entire colony, in a manner that can be applied to any potentially harmful chemicals or pathogens. A technique enabling a better understanding of the challenges facing these critical pollinators leaves us buzzing.

    From food-producing pollinators to clean water...in JoVE Bioengineering this month, our Authors present methods to prepare low-cost, efficient membranes for water purification. Here, zwitterionic hydrogels are produced using SBMA* crosslinked to polyethylene glycol diacrylate to improve mechanical strength. When impregnated into a highly porous hydrophobic support, the resultant tightly bound layers prevent antifouling of the membranes by physically preventing attachment to the surface. These properties combined allow for production of energy efficient and long lasting filters to meet increasing demand for clean water. Something we can all toast to, cheers!

    In JoVE Behavior, our Authors demonstrate a procedure of exposure manipulation involving sorting novel and familiar objects paired with an acoustic signal, to reveal the powerful shaping role of experience in recognizing human language. At 3-4 months infants broadly recognize both human and nonhuman primate vocalizations as cues to support object categorization, but by 6-to 7-months only human vocalizations are utilized. This method shows that merely exposing infants to nonhuman primate vocalizations can preserve this early-established link, whereas the same effect is not observed when the child is exposed to backwards speech.

    Finally, as the first buds of spring emerge, our last video highlight this month gives a fascinating glimpse into the private life of plants. In JoVE Developmental Biology, our Author uses confocal microscopy to show how the intricate inflorescences of the Arabidopsis plant develop in real time. Moreover, the demonstrated procedure allows expression of multiple genes to be visualized at once, aiding in the elucidation of the complex genetic networks, which unravel simultaneously to specify the identities of the different floral organs. Blooming marvelous!

    You've just had a sneak peek of the April 2017 issue of JoVE. Visit the website to see the full-length articles, plus many more, in JoVE: The World's Premier Video Journal.

    Protocol

    Experience is Instrumental in Tuning a Link Between Language and Cognition: Evidence from 6- to 7- Month-Old Infants' Object Categorization

    Danielle Perszyk, Sandra Waxman

    Psychology Department, Northwestern University

    At 3-4 months, listening to human and nonhuman primate vocalizations boosts infants' cognition; by 6 months, only human vocalizations exert this cognitive advantage. We describe an exposure manipulation that reveals the powerful shaping role of experience as infants specify which sounds to link to cognition and which to tune out.

    Live Confocal Imaging of Developing Arabidopsis Flowers

    Nathanaël Prunet

    Department of Biology and Biological Engineering, California Institute of Technology

    Live confocal imaging provides biologists with a powerful tool to study development. Here, we present a detailed protocol for the live confocal imaging of developing Arabidopsis flowers.

    Evaluating the Effect of Environmental Chemicals on Honey Bee Development from the Individual to Colony Level

    Chong-Yu Ko, Yue-Wen Chen, Yu-Shin Nai

    Department of Biotechnology and Animal Science, National Ilan University

    Herein we present a method to feed pesticide contaminated food to both an individual honey bee and a beehive colony. The procedure evaluates the pesticide effect on individual honey bees by in vivo feeding of basic larval diet and also on the natural condition of beehive colony.

    Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification

    Thien N. Tran, Sankara N. Ramanan, Haiqing Lin

    Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York at Buffalo

    This paper reports practical methods to prepare hydrogels in freestanding films and impregnated membranes and to characterize their physical properties, including water transport properties.

    Disclosures

    No conflicts of interest declared.

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