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Cultivo y manipulación de células de la cresta Neural O9-1

Article DOI: 10.3791/58346-v • 08:32 min • October 9th, 2018
October 9th, 2018 •
1, 2, 2, 1
1Molecular Physiology and Biophysics, Baylor College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern California

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O9-1 es una línea de células de cresta neural ratón pluripotentes. Aquí describimos protocolos detallados paso a paso para cultivar células O9-1, diferenciar células O9-1 hacia tipos celulares específicos y manipular genéticamente las células O9-1 mediante caída mediada por siRNA o CRISPR Cas9 edición del genoma.

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Genética número 140 CRISPR Cas9 O9-1 células golpe de gracia del gene gene precipitación nervios de la cresta
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