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JoVE Journal Genetics
Kultivierung und Manipulation von Zellen der Neuralleiste O9-1
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Kultivierung und Manipulation von Zellen der Neuralleiste O9-1

Article DOI: 10.3791/58346-v • 08:32 min • October 9th, 2018
October 9th, 2018 •
1, 2, 2, 1
1Molecular Physiology and Biophysics, Baylor College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern California

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O9-1 ist eine Zell-Linie multipotent Maus Neuralleiste. Hier beschreiben wir detaillierte Schritt für Schritt Protokolle für die Kultivierung O9-1-Zellen, O9-1-Zellen in bestimmten Zelltypen differenzieren und genetisch manipuliert O9-1-Zellen mit SiRNA-vermittelten Zuschlag oder CRISPR-Cas9 Genom-Bearbeitung.

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Genetik Ausgabe 140 CRISPR Cas9 O9-1 Zellen Gene Knockout gen-Knockdown Neuralleiste Zellen
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