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Genetics

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JoVE Journal Genetics
Dyrking og manipulering av O9-1 Neural Crest celler
Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
Journal (Genetics)
Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
Generation and Purification of Chromosome Conformation Capture (3C) Library (Video) | JoVE
Journal (Genetics)
Generation and Purification of Chromosome Conformation Capture (3C) Library (Video) | JoVE
 
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Dyrking og manipulering av O9-1 Neural Crest celler

Article DOI: 10.3791/58346-v • 08:32 min • October 9th, 2018
October 9th, 2018 •
1, 2, 2, 1
1Molecular Physiology and Biophysics, Baylor College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern California

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O9-1 er en multipotent musen neural crest cellen linje. Her beskriver vi detaljerte trinnvise protokoller for dyrking O9-1 cellene skille O9-1 celler i spesifikke celletyper og genetisk manipulering O9-1 celler ved hjelp av siRNA-mediert knockdown eller CRISPR-Cas9 genomet redigering.

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Genetikk problemet 140 CRISPR Cas9 O9-1 celler gene knockout gene knockdown neural crest celler
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