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MUB40 peptid til brug i forbindelse med afsløring neutrofile-medieret betændelse begivenheder
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MUB40 peptid til brug i forbindelse med afsløring neutrofile-medieret betændelse begivenheder

Article DOI: 10.3791/58367-v • 06:48 min • January 7th, 2019
January 7th, 2019 •
1,2, 1,2,3, 1,2,3, 1,2,4, 1,2
1Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, 2INSERM Unité 1202, Institut Pasteur, 3Institut de Biochimie et Génétique Cellulaires, Université Bordeaux Segalen, 4Unit of Technology and Service Photonic BioImaging, Institut Pasteur

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Vi præsenterer her, en protokol for at påvise tilstedeværelsen af neutrofiler i fast/permeabilized histologi sektioner og vurdere aktiveringstilstand for levende renset neutrofiler. Især binder MUB40 peptid lactoferrin stede i neutrofile-specifikke og tertiære granulat. Eksponering af granulet indholdet gennem enten permeabilization eller neutrofile aktivering giver mulighed for mærkning af neutrofiler.

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Biologi spørgsmålet 143 MUB40 peptid retro-inverso neutrofile betændelse biomarkør Laktoferrin i vivo
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