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Syntes och strukturbestämning av µ-Conotoxin PIIIA isomerer med olika disulfid Connectivities
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Syntes och strukturbestämning av µ-Conotoxin PIIIA isomerer med olika disulfid Connectivities

Article DOI: 10.3791/58368-v • 11:44 min • October 2nd, 2018
October 2nd, 2018 •
*1, *1, *1, 1
1Pharmaceutical Biochemistry and Bioanalytics, Pharmaceutical Institute, University of Bonn
* These authors contributed equally

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Cystein-rika peptider vik distinkta tredimensionella strukturer beroende på deras disulfid anslutning. Riktade syntes av enskilda disulfid isomerer krävs när bufferten oxidation inte leder till önskad disulfid anslutning. Protokollet behandlar selektiv syntesen av 3-disulfid-bundna peptider och deras strukturella analys med hjälp av NMR- och MS/MS studier.

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Kemi fråga 140 peptider cystein-rika disulfid connectivity syntes skydda koncernens strategi strukturanalys 2D NMR (kärnmagnetisk resonans) MS/MS (tandem mass spectrometry)
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