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JoVE Journal Neuroscience
Electrophysiological Recording of The Central Nervous System Activity of Third-Instar Drosophila Melanogaster
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Electrophysiological Recording of The Central Nervous System Activity of Third-Instar Drosophila Melanogaster

Article DOI: 10.3791/58375-v • 06:45 min • November 21st, 2018
November 21st, 2018 •
1, 2, 3, 3
1Department of Entomology, Louisiana State University AgCenter, 2Department of Entomology, Virginia Tech, 3Department of Entomology and Nematology, Emerging Pathogens Institute, University of Florida

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Summary
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This protocol describes a method to record the descending electrical activity of the Drosophila melanogaster central nervous system to enable the cost-efficient and convenient testing of pharmacological agents, genetic mutations of neural proteins, and/or the role of unexplored physiological pathways.

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Electrophysiological Recording Central Nervous System Activity Third-instar Drosophila Melanogaster Insect Toxicology Insect Physiology Electrogenesis Scientific Hypotheses Insecticide Modes Of Action Technique Simple Reproducible High-throughput System Nervous System Study Acquisition/analysis Software Channel Settings Raw Signal Recording Event Counting Threshold Measurement Detection Settings Electrical Activity Conversion
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