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JoVE Journal Neuroscience
Isolamento dei Nuclei del midollo spinale adulto per il sequenziamento massivamente parallelo Single-nucleo RNA
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Isolamento dei Nuclei del midollo spinale adulto per il sequenziamento massivamente parallelo Single-nucleo RNA

Article DOI: 10.3791/58413-v • 06:38 min • October 12th, 2018
October 12th, 2018 •
1, 1, 2, 3,4, 3, 1
1Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke, 2Bioinformatics Section, Information Technology Program, National Institute of Neurological Disorders and Stroke, 3Laboratory of Cochlear Development, National Institute on Deafness and Other Communication Disorders, 4Single Cell Analysis Facility, Frederick National Laboratory

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Qui, presentiamo un protocollo per isolare rapidamente i nuclei di alta qualità dal tessuto fresco o congelato per sequenziamento di RNA massicciamente parallelo a valle. Includiamo opzioni lisi delle cellule e la rottura detergente-meccanica e ipotonico-meccanica del tessuto, entrambi dei quali possono essere utilizzati per l'isolamento dei nuclei.

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Neuroscienze problema 140 Nuclei RNA sequencing snRNA-Seq parallelismo massivo midollo spinale gradiente di saccarosio
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