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Journal / Neuroscience / Yetişkin omurilik çekirdeği yalıtım kitlesel…
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Yetişkin omurilik çekirdeği yalıtım kitlesel paralel tek-çekirdek RNA sıralama için
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Yetişkin omurilik çekirdeği yalıtım kitlesel paralel tek-çekirdek RNA sıralama için

Article DOI: 10.3791/58413-v • 06:38 min • October 12th, 2018
October 12th, 2018 •
1, 1, 2, 3,4, 3, 1
1Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke, 2Bioinformatics Section, Information Technology Program, National Institute of Neurological Disorders and Stroke, 3Laboratory of Cochlear Development, National Institute on Deafness and Other Communication Disorders, 4Single Cell Analysis Facility, Frederick National Laboratory

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Burada, hızlı bir şekilde yüksek kaliteli çekirdekleri aşağı akım kitlesel paralel RNA sıralama için taze veya dondurulmuş dokusundan izole etmek için bir iletişim kuralı mevcut. İkisi de çekirdek yalıtımı için kullanılan deterjan-mekanik ve mekanik hipotonik doku bozulma ve hücre lizis seçenekleri, içerir.

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Neuroscience sayı: 140 çekirdek sıralama snRNA Seq kitlesel paralel spinal kord sukroz degrade RNA
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