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Journal / Neuroscience / Use of Pre-Assembled Plastic Microfluidic Chips…
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Neuroscience

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JoVE Journal Neuroscience
Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons
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Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons

Article DOI: 10.3791/58421-v • 10:50 min • November 2nd, 2018
November 2nd, 2018 •
1,2, 3, 3, 1,2,3
1UNC Neuroscience Center, 2UNC/NC State Joint Department of Biomedical Engineering, UNC, 3Xona Microfluidics, LLC

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Summary
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This protocol describes the use of plastic chips to culture and compartmentalize primary murine neurons. These chips are preassembled, user-friendly, and compatible with high-resolution, live, and fluorescence imaging. This protocol describes how to plate rat hippocampal neurons within these chips and perform fluidic isolation, axotomy and immunostaining.

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Pre-assembled Plastic Microfluidic Chips Compartmentalizing Neurons Neuroscience Subcellular Manipulation Culture Devices Axon Development Synapse Remodeling Alzheimer's Disease Primary Rat Neurons Reproducible Method Silicon-based Devices High Resolution Microscopy Human Stem Cells Multicompartment Chip
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