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Journal / Genetics / Omvendt transkripsjon-Loop-mediert isotermiske…
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JoVE Journal Genetics
Omvendt transkripsjon-Loop-mediert isotermiske forsterkning (RT-lampe) analysen for Zika Virus og Housekeeping gener i urin, Serum og mygg prøver
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Omvendt transkripsjon-Loop-mediert isotermiske forsterkning (RT-lampe) analysen for Zika Virus og Housekeeping gener i urin, Serum og mygg prøver

Article DOI: 10.3791/58436-v • 08:03 min • September 14th, 2018
September 14th, 2018 •
1, 2, 2, 1,3, 1,3
1Department of Urology, Beaumont Health System, 2Foundational Sciences, College of Medicine, Central Michigan University, 3Oakland University William Beaumont School of Medicine

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Denne protokollen gir en effektiv og rimelig metode for å oppdage Zika virus eller kontrollere mål i prøver fra mennesker urin og serum eller mygg av omvendt transkripsjon loop-mediert isotermiske forsterkning (RT-lampe). Denne metoden krever ikke RNA isolasjon og kan gjøres innen 30 min.

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Genetikk problemet 139 Zika Virus RT-lampe urin serum Aedes point-of-care testing feltet verktøy
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