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Journal / Biochemistry / At studere Protein Import til grønkorn ved hjælp…
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JoVE Journal Biochemistry
At studere Protein Import til grønkorn ved hjælp af Protoplasts
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At studere Protein Import til grønkorn ved hjælp af Protoplasts

Article DOI: 10.3791/58441-v • 06:29 min • December 10th, 2018
December 10th, 2018 •
1, 1, 1,2
1Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, 2Department of Life Sciences, Pohang University of Science and Technology

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Her beskriver vi en protokol for at udtrykke proteiner i protoplasts ved hjælp af PEG-medieret transformation metode. Metoden giver nem udtryk af proteiner af interesse og effektiv undersøgelse af protein lokalisering og importprocessen for forskellige eksperimentelle betingelser in vivo.

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Biokemi sag 142 Protoplasts kloroplaster Arabidopsis protein import polyethylenglycol forbigående udtryk
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