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Immunology and Infection

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JoVE Journal Immunology and Infection
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Immunizzazione di Zebrafish adulto per lo Screening preclinico di vaccini basati sul DNA

Article DOI: 10.3791/58453-v • 05:39 min • October 30th, 2018
October 30th, 2018 •
*1, *1, 1,2, 1,3,4,5
1BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, 2MedEngine Oy, 3Department of Pediatrics, Tampere University Hospital, 4Department of Children and Adolescents, Oulu University Hospital, 5PEDEGO Research Unit, Medical Research Center, University of Oulu
* These authors contributed equally

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Qui descriviamo un protocollo per l'immunizzazione del adulto zebrafish (Danio rerio) con un vaccino basato su DNA e dimostrare la convalida di un evento di successo vaccinazione. Questo metodo è adatto per lo screening preclinico di candidati vaccinali in vari modelli di infezione.

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Immunologia e infezione problema 140 vaccino a DNA vaccinazione zebrafish fluorescenza plasmide pCMV-GFP microinjector intramuscolare pre-clinico di screening modello animale micobatteri antigene immunizzazione
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