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Journal / Immunology and Infection / Immunisering av voksen sebrafisk for prekliniske…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Immunisering av voksen sebrafisk for prekliniske Screening av DNA-baserte vaksiner
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Immunisering av voksen sebrafisk for prekliniske Screening av DNA-baserte vaksiner

Article DOI: 10.3791/58453-v • 05:39 min • October 30th, 2018
October 30th, 2018 •
*1, *1, 1,2, 1,3,4,5
1BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, 2MedEngine Oy, 3Department of Pediatrics, Tampere University Hospital, 4Department of Children and Adolescents, Oulu University Hospital, 5PEDEGO Research Unit, Medical Research Center, University of Oulu
* These authors contributed equally

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Her beskriver vi en protokoll for immunisering av voksen sebrafisk (Danio rerio) med en DNA-baserte vaksine og demonstrere validering av en vellykket vaksinasjon hendelse. Denne metoden er egnet for prekliniske screening av vaksine kandidater i ulike infeksjon modeller.

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Immunologi og infeksjon problemet 140 DNA-vaksine vaksine sebrafisk fluorescens bildebehandling pCMV-GFP plasmider microinjector intramuskulær pre-klinisk screening dyremodell mykobakterier antigen immunisering
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