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Journal / Genetics / Fare embriyonik kök hücre kullanarak Southern…
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JoVE Journal Genetics
Fare embriyonik kök hücre kullanarak Southern blot ve polimeraz zincir reaksiyonu Homolog rekombinasyon olaylar tanımlaması
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Fare embriyonik kök hücre kullanarak Southern blot ve polimeraz zincir reaksiyonu Homolog rekombinasyon olaylar tanımlaması

Article DOI: 10.3791/58467-v • 08:01 min • November 20th, 2018
November 20th, 2018 •
*1,2, *1, *3, 1, 1, 1, 4, 5, 3, 1
1Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine, Hunan Agricultural University (HUNAU), 2Department of Pathology, Georgetown University Medical School, 3College of Food Science and Technology, Hunan Agricultural University (HUNAU), 4Lab of Molecular Cardiology (LMC), National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH), 5Transgenic Core, National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)
* These authors contributed equally

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Burada, fare embriyonik kök Southern blot ve/veya PCR kullanarak hücre Homolog rekombinasyon olayları tanımlamak için detaylı bir iletişim kuralı mevcut. Bu yöntem ile nonmuscle myosin II genetik değiştirme fare modelleri geleneksel embriyonik kök hücre tabanlı Homolog rekombinasyon aracılı hedefleme teknolojisi kullanarak oluşturma tarafından örneklenir.

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Genetik sayı: 141 Homolog rekombinasyon gen hedefleme Southern Blot PCR fare embriyonik kök hücre sonda astar genetik değişimi nakavt/knock-in Myh9 gene genomik DNA
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