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Journal / Genetics / Identifikation af homologe rekombination…
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Genetics

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JoVE Journal Genetics
Identifikation af homologe rekombination begivenheder i mus embryonale stamceller ved hjælp af det sydlige Blotting og Polymerase kædereaktion
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Identifikation af homologe rekombination begivenheder i mus embryonale stamceller ved hjælp af det sydlige Blotting og Polymerase kædereaktion

Article DOI: 10.3791/58467-v • 08:01 min • November 20th, 2018
November 20th, 2018 •
*1,2, *1, *3, 1, 1, 1, 4, 5, 3, 1
1Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine, Hunan Agricultural University (HUNAU), 2Department of Pathology, Georgetown University Medical School, 3College of Food Science and Technology, Hunan Agricultural University (HUNAU), 4Lab of Molecular Cardiology (LMC), National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH), 5Transgenic Core, National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)
* These authors contributed equally

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Vi præsenterer her, en detaljeret protokol for at identificere homologe rekombination begivenheder, der fandt sted i mus embryonale stamceller ved hjælp af det sydlige blotting og/eller PCR. Denne metode er eksemplificeret ved generation af nonmuscle myosin II genetiske udskiftning musemodeller ved hjælp af traditionelle embryonale stamcelle-baseret homologe rekombination-medieret målretning teknologi.

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Genetik sag 141 homologe rekombination gen målretning sydlige blotting PCR mus embryonale stamceller sonde primer genetiske udskiftning knockout/knock-i Myh9 gen genomisk DNA
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