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Journal / Genetics / Identifikation der homologen…
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Genetics

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JoVE Journal Genetics
Identifikation der homologen Rekombinationsereignisse in embryonalen Stammzellen der Maus mit Southern Blotting und Polymerase-Kettenreaktion
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Identifikation der homologen Rekombinationsereignisse in embryonalen Stammzellen der Maus mit Southern Blotting und Polymerase-Kettenreaktion

Article DOI: 10.3791/58467-v • 08:01 min • November 20th, 2018
November 20th, 2018 •
*1,2, *1, *3, 1, 1, 1, 4, 5, 3, 1
1Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine, Hunan Agricultural University (HUNAU), 2Department of Pathology, Georgetown University Medical School, 3College of Food Science and Technology, Hunan Agricultural University (HUNAU), 4Lab of Molecular Cardiology (LMC), National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH), 5Transgenic Core, National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)
* These authors contributed equally

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Hier präsentieren wir Ihnen ein detailliertes Protokoll zur Identifizierung von homologen Rekombinationsereignisse in embryonalen Stammzellen der Maus mit Southern blotting und/oder PCR. Diese Methode ist durch die Generierung von Nonmuscle Myosin II genetische Ersatz Mausmodellen mit traditionellen embryonalen Stammzell-basierte homologe Rekombination-vermittelten targeting-Technologie veranschaulicht.

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Genetik Ausgabe 141 homologe Rekombination Gene targeting,-Southern Blot PCR Maus embryonale Stammzellen Sonde Grundierung genetischen Austausch Ko/Knock-in Myh9 gen genomische DNA
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