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JoVE Journal Neuroscience
Preparazione delle fette Acute del midollo spinale per la registrazione di cellule intere Patch-clamp in neuroni di sostanza Gelatinosa
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Preparazione delle fette Acute del midollo spinale per la registrazione di cellule intere Patch-clamp in neuroni di sostanza Gelatinosa

Article DOI: 10.3791/58479-v • 08:30 min • January 18th, 2019
January 18th, 2019 •
1, 1, 2, 2, 2, 2, 2,3
1Department of Pain, First Affiliated Hospital of Nanchang University, 2Department of Pediatrics, First Affiliated Hospital of Nanchang University, 3Center for Laboratory Medicine, First Affiliated Hospital of Nanchang University

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Qui, descriviamo i passi essenziali per le registrazioni di cellule intere patch-clamp fatta da neuroni di sostanza gelatinosa (SG) nella fetta di midollo spinale in vitro . Questo metodo consente la proprietà intrinseca della membrana, la trasmissione sinaptica e la caratterizzazione morfologica dei neuroni di SG per essere studiato.

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Neuroscienze problema 143 neuroscienze fetta di midollo spinale sostanza gelatinosa neurone cellule intere patch-clamp elettrofisiologia morfologia in vitro
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