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A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci

Article DOI: 10.3791/58502-v • 05:03 min • October 29th, 2018
October 29th, 2018 •
1,2,3, 4, 4, 1, 5, 6,7, 6, 2,3, 2,3, 1, 1, 8
1Department of Method Development and Analytics, Agroscope, 2Swiss Tropical and Public Health Institute, 3University of Basel, 4Swiss Federal Plant Protection Service, Federal Office for Agriculture, 5OptiGene Limited, 6Fera Science Limited, 7School of Natural and Environmental Sciences, Newcastle University, 8Department of Plants and Plant Products, Agroscope

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This paper reports the protocol for a rapid identification assay for Bemisia tabaci based on loop-mediated isothermal amplification (LAMP) technology. The protocol requires minimal laboratory training and can, therefore, be implemented on-site at points of entry for plant imports such as seaports and airports.

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Loop-mediated Isothermal Amplification (LAMP) Assay Bemisia Tabaci Invasive Insect Pest Vegetable Crops Ornamental Crops Diagnostic Tool On-site Testing Plant Import Products Trading Networks LAMP Strip Label Tubes B.tabaci LAMP Reaction Master Mix Centrifuge PAC (positive Amplification Control) Ready-to-use B.tabaci LAMP Kit Units DNA Extraction Insect Specimens DNA Extraction Solution ThermoMixer
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