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Journal / Immunology and Infection / Samtidige studie av rekrutteringen av Monocyte…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Samtidige studie av rekrutteringen av Monocyte subpopulasjoner Under flyt In Vitro
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Samtidige studie av rekrutteringen av Monocyte subpopulasjoner Under flyt In Vitro

Article DOI: 10.3791/58509-v • 09:16 min • November 26th, 2018
November 26th, 2018 •
1, 2, 1, 3, 3
1Hematology service, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva, 2Department of Pathology and Immunology, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva, 3Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva

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Her presenterer vi en integrert protokoll som måler monocytt subpopulasjon smugling under flyt i vitro ved bruk av bestemte overflate markører og AC confocal fluorescens mikroskopi. Denne protokollen kan brukes å utforske sekvensiell rekruttering trinn også om profil andre leukocytter subtyper med andre spesifikke overflate markører.

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Immunologi og infeksjon problemet 141 leukocytter menneskehandel monocyte rekruttering betennelse timelapse tenkelig AC confocal mikroskopi vedheft transmigration monocyte subpopulasjoner chemokines integrins vedheft molekyler
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