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Journal / Immunology and Infection / Samtidige undersøgelse af ansættelse af monocyt…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Samtidige undersøgelse af ansættelse af monocyt delpopulationer Under Flow In Vitro
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Samtidige undersøgelse af ansættelse af monocyt delpopulationer Under Flow In Vitro

Article DOI: 10.3791/58509-v • 09:16 min • November 26th, 2018
November 26th, 2018 •
1, 2, 1, 3, 3
1Hematology service, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva, 2Department of Pathology and Immunology, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva, 3Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva

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Vi præsenterer her, en integreret protokol, der måler monocyt delpopulation handel under flow in vitro-ved brug af specifikke overflade markører og konfokal Fluorescens mikroskopi. Denne protokol kan bruges til at udforske sekventielle rekruttering trin samt for at profilere andre leukocyt undertyper ved hjælp af andre specifikke overflade markører.

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Immunologi og infektion sag 141 leukocyt handel monocyt rekruttering betændelse timelapse imaging Konfokal mikroskopi vedhæftning transmigration monocyt delpopulationer kemokiner integriner adhæsionsmolekyler
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