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Journal / Immunology and Infection / Samtidiga studie av rekrytering av monocyt…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Samtidiga studie av rekrytering av monocyt Subpopulations Under flöde In Vitro
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Samtidiga studie av rekrytering av monocyt Subpopulations Under flöde In Vitro

Article DOI: 10.3791/58509-v • 09:16 min • November 26th, 2018
November 26th, 2018 •
1, 2, 1, 3, 3
1Hematology service, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva, 2Department of Pathology and Immunology, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva, 3Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva

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Här presenterar vi ett integrerat protokoll som mäter monocyt subpopulation människohandel under flöde in vitro-användning av specifika yta markörer och confocal fluorescensmikroskopi. Detta protokoll kan användas för att utforska sekventiell rekrytering steg samt för att profilera andra leukocyt subtyper med andra specifika yta markörer.

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Immunologi och infektion fråga 141 leukocyt människohandel monocyt rekrytering inflammation timelapse bildskapande confocal microscopy vidhäftning själavandring monocyt subpopulations chemokiner integriner adhesionsmolekyler
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