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JoVE Journal Neuroscience
Одновременно Cryosectioning нескольких грызунов мозги
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Одновременно Cryosectioning нескольких грызунов мозги

Article DOI: 10.3791/58513-v • 06:37 min • September 18th, 2018
September 18th, 2018 •
1,2,3, 1, 5, 1,3,4, 1,3,4
1Department of Child Health, University of Arizona College of Medicine, 2Department of Biological Sciences, University of Bath, 3BARROW Neurological Institute at Phoenix Children's Hospital, 4Phoenix Veteran Affairs Healthcare System, 5Department of Basic Medical Sciences, University of Arizona College of Medicine

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Здесь мы представляем протокол к замораживанию и раздел мозговой ткани из нескольких животных как экономия времени альтернатива для обработки одного мозги. Это уменьшает окрашивание изменчивость во время иммуногистохимии и уменьшает время cryosectioning и томографии.

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Нейробиологии выпуск 139 неврологии гистология иммуногистохимия грызунов криозащиты cryosectioning криостат невропатология
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