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JoVE Journal Neuroscience
मल्टीपल कुतर दिमाग का एक साथ Cryosectioning
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मल्टीपल कुतर दिमाग का एक साथ Cryosectioning

Article DOI: 10.3791/58513-v • 06:37 min • September 18th, 2018
September 18th, 2018 •
1,2,3, 1, 5, 1,3,4, 1,3,4
1Department of Child Health, University of Arizona College of Medicine, 2Department of Biological Sciences, University of Bath, 3BARROW Neurological Institute at Phoenix Children's Hospital, 4Phoenix Veteran Affairs Healthcare System, 5Department of Basic Medical Sciences, University of Arizona College of Medicine

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यहाँ, हम एक timesaving एक दिमाग प्रसंस्करण के लिए विकल्प के रूप में कई जानवरों से फ्रीज और अनुभाग मस्तिष्क ऊतक के लिए एक प्रोटोकॉल मौजूद. यह immunohistochemistry के दौरान धुंधला परिवर्तनशीलता कम कर देता है और समय cryosectioning और इमेजिंग कम कर देता है ।

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तंत्रिका विज्ञान अंक १३९ तंत्रिका विज्ञान प्रोटोकॉल immunohistochemistry मूषक cryoprotection cryosectioning cryostat neuropathology
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