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Journal / Biochemistry / Affinitet rensning af grønkorn Translocon Protein …
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Biochemistry

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JoVE Journal Biochemistry
Affinitet rensning af grønkorn Translocon Protein komplekser ved hjælp af tryk på Tag
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Affinitet rensning af grønkorn Translocon Protein komplekser ved hjælp af tryk på Tag

Article DOI: 10.3791/58532-v • 07:01 min • November 1st, 2018
November 1st, 2018 •
1, 1, 2, 1
1Laboratory of Plant Physiology, University of Neuchatel, 2Institut fur Biochemie und Biotechnologie, Martin-Luther-Universitat Halle-Wittenberg

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Her præsenterer vi en gennemprøvet og testet protokol til rensning af grønkorn protein import komplekser (TOC-TIC kompleks) ved hjælp af tryk-tag. Et-trins affinitet-isolation protokollen kan potentielt anvendes til enhver protein og bruges til at identificere nye interaktion partnere ved massespektrometri.

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Biokemi sag 141 grønkorn TOC-TIC komplekse TAP tag TOC159 IgG bindende TEV protease affinitet rensning.
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