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Journal / Biochemistry / Affinity Purification of Chloroplast Translocon…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
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Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag

Article DOI: 10.3791/58532-v • 07:01 min • November 1st, 2018
November 1st, 2018 •
1, 1, 2, 1
1Laboratory of Plant Physiology, University of Neuchatel, 2Institut fur Biochemie und Biotechnologie, Martin-Luther-Universitat Halle-Wittenberg

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Summary
Automatic Translation

We here present a proven and tested protocol for the purification of chloroplast protein import complexes (TOC-TIC complex) using the TAP-tag. The one-step affinity-isolation protocol can potentially be applied to any protein and be used to identify new interaction partners by mass spectrometry.

Tags

Chloroplast Translocon Protein Complexes TAP Tag Chloroplast Protein Import Machinery TOC Complex TIC Complex Purification Method TAP-TOC Purification Arabidopsis Plants Grinding Buffer Filtration Material Centrifugation Supernatant
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