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Journal / Biochemistry / Affinitet rensing av Chloroplast Translocon…
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Biochemistry

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JoVE Journal Biochemistry
Affinitet rensing av Chloroplast Translocon Protein komplekser hjelp av trykk-koden
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Affinitet rensing av Chloroplast Translocon Protein komplekser hjelp av trykk-koden

Article DOI: 10.3791/58532-v • 07:01 min • November 1st, 2018
November 1st, 2018 •
1, 1, 2, 1
1Laboratory of Plant Physiology, University of Neuchatel, 2Institut fur Biochemie und Biotechnologie, Martin-Luther-Universitat Halle-Wittenberg

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Vi presenterer her en utprøvd og testet protokoll for rensing av chloroplast protein import komplekser (TOC-TIC komplekse) bruke trykk-koden. Ett-trinns affinitet-isolasjon protokollen kan potensielt brukes til alle protein og brukes til å identifisere nye samhandling partnere av massespektrometri.

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Biokjemi problemet 141 Chloroplast TOC-TIC komplekse trykk tag TOC159 IgG bindende TEV protease affinitet rensing.
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