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Biochemistry

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JoVE Journal Biochemistry
Isolamento dei Glomeruli e In Vivo di etichettatura delle proteine di superficie delle cellule glomerulari
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Isolamento dei Glomeruli e In Vivo di etichettatura delle proteine di superficie delle cellule glomerulari

Article DOI: 10.3791/58542-v • 09:12 min • January 18th, 2019
January 18th, 2019 •
1, 1, 1, 2, 1, 1, 1, 1, 1, 1
1Department of Nephrology, Medical Faculty, Heinrich-Heine-University, 2Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf

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Qui presentiamo un protocollo per l'etichettatura dei Murina in vivo delle proteine di superficie delle cellule glomerulari con biotina. Questo protocollo contiene informazioni su come irrorare i reni del mouse, isolare i glomeruli ed eseguire immunoprecipitazione endogena della proteina di interesse.

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Biochimica problema 143 In vivo biotinylation etichettatura di proteina di superficie delle cellule dei glomeruli isolamento dei glomeruli biglie magnetiche endocitosi in vivo traffico della proteina
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