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Journal / Medicine / 体外培养肺泡上皮细胞中卤代气体浓度的控制方法…
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JoVE Journal Medicine
体外培养肺泡上皮细胞中卤代气体浓度的控制方法
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体外培养肺泡上皮细胞中卤代气体浓度的控制方法

Article DOI: 10.3791/58554-v • 04:56 min • October 23rd, 2018
October 23rd, 2018 •
1,2, 1,2, 3, 2,4, 2,4, 2,4, 2,4, 2, 2, 1,2, 1,2, 1,2, 2, 1,2
1Department of Perioperative Medicine, CHU Clermont-Ferrand, 2Centre National de la Recherche Scientifique Unité Mixte de Recherche (CNRS UMR) 6293, Institut National de la Santé et de la Recherche Médicale (INSERM) U1103, Laboratoire de Génétique, Reproduction et Développement (GReD), Université Clermont Auvergne, 3Department of Pharmacology, CHU Clermont-Ferrand, 4Nurse Anesthetist School, CHU Clermont-Ferrand

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我们描述了一个简单的协议, 专门设计, 以达到精确和控制的浓度七氟醚或异氟醚在体外, 以提高我们对介入的机制在上皮性肺损伤的理解和测试小说急性呼吸窘迫综合征的治疗。

Tags

医学 140 期 细胞培养 七氟醚 异氟烷 卤代气体 肺泡上皮细胞 肺 ARDS 气液界面 色谱
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