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Journal / Genetics / Eine anpassbare Protokoll für String Montage gRNA …
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Genetics

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JoVE Journal Genetics
Eine anpassbare Protokoll für String Montage gRNA Klonen (STAgR)
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Eine anpassbare Protokoll für String Montage gRNA Klonen (STAgR)

Article DOI: 10.3791/58556-v • 10:00 min • December 26th, 2018
December 26th, 2018 •
1,2, 1,2, 3, 3, 2,4, 1,2
1MCN Junior Research Group, Munich Center for Neurosciences, Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum, German Research Center for Environmental Health, 4Physiological Genomics, Ludwig Maximilian Universitat, BioMedical Center

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Hier präsentieren wir Zeichenfolge Montage gRNA Klonen (STAgR), eine Methode, um leicht gRNA Vektoren multiplex für nähert sich CRISPR/Cas9. STAgR macht gRNA Multiplexen, einfach, effizient und individuell.

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Genetik Ausgabe 142 CRISPR Cas9 dCas9 gRNA Klonen gRNA multiplexing Genom Bearbeitung Transkriptom bearbeiten
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