JoVE Journal
Genetics
Genetics
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文字列アセンブリ gRNA のカスタマイズ可能なプロトコル (STAgR) のクローニング
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Article
DOI:
10.3791/58556-v
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10:00 min
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December 26th, 2018
文字列アセンブリ gRNA のカスタマイズ可能なプロトコル (STAgR) のクローニング
December 26th, 2018
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Christopher T. Breunig1,2, Andrea M. Neuner1,2, Jessica Giehrl-Schwab3, Wolfgang Wurst3, Magdalena Götz2,4, Stefan H. Stricker1,2
1MCN Junior Research Group, Munich Center for Neurosciences, Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum, German Research Center for Environmental Health, 4Physiological Genomics, Ludwig Maximilian Universitat, BioMedical Center
Chapters
Summary
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文字列アセンブリ gRNA (STAgR) のクローニングを紹介ここでは、簡単に CRISPR/Cas9 のアプローチの gRNA のベクトルを多重化する方法。STAgR は、シンプルで効率的かつカスタマイズ可能な gRNA 多重になります。