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Journal / Biology / LDL-Cholesterin-Aufnahme-Assay mit Live Cell…
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Biology

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LDL-Cholesterin-Aufnahme-Assay mit Live Cell Imaging-Analyse mit Zelle Systemüberwachung
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LDL-Cholesterin-Aufnahme-Assay mit Live Cell Imaging-Analyse mit Zelle Systemüberwachung

Article DOI: 10.3791/58564-v • 08:45 min • November 17th, 2018
November 17th, 2018 •
*1,2, *1,3, 4, 4,5, 6, 1,2,7,8
1Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, 2Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, 3Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, 4Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami Leonard M. Miller School of Medicine, 5John P. Hussman Institute for Human Genomics, University of Miami Leonard M. Miller School of Medicine, 6Department of Medicine, Division of Endocrinology, Metabolism and Endocrinology and the Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, 7Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, 8Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine
* These authors contributed equally

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Summary
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Dieses Protokoll bietet einen effizienten Ansatz zur Messung von LDL-Cholesterin-Aufnahme mit Real-Time Zustrom Raten mit einem live Cell imaging-System in verschiedenen Zelltypen. Diese Technik bietet eine Plattform die pharmakologische Aktivität von Verbindungen beeinflussen LDL Zustrom während der Überwachung für Zellmorphologie und damit potenzielle Zytotoxizität auf dem Bildschirm.

Tags

Biologie Ausgabe 141 Low Density Lipoprotein LDLR LDL Zustrom LDL-Aufnahme Dynasore Simvastatin rekombinante PCSK9 Dynamin Lipide Cholesterin Arteriosklerose.
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