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Journal / Biology / LDL-kolesterol upptag Assay använder levande Cell …
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Biology

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LDL-kolesterol upptag Assay använder levande Cell Imaging analys med Cell hälsoövervakning
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LDL-kolesterol upptag Assay använder levande Cell Imaging analys med Cell hälsoövervakning

Article DOI: 10.3791/58564-v • 08:45 min • November 17th, 2018
November 17th, 2018 •
*1,2, *1,3, 4, 4,5, 6, 1,2,7,8
1Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, 2Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, 3Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, 4Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami Leonard M. Miller School of Medicine, 5John P. Hussman Institute for Human Genomics, University of Miami Leonard M. Miller School of Medicine, 6Department of Medicine, Division of Endocrinology, Metabolism and Endocrinology and the Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, 7Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, 8Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine
* These authors contributed equally

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Summary
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Detta protokoll ger en effektiv metod för att mäta LDL kolesterol upptag med realtid tillströmning priser med hjälp av en levande cell imaging system i olika celltyper. Denna teknik ger en plattform för att skärmen den farmakologiska aktiviteten av föreningar som påverkar LDL tillströmning medan övervakning för cellmorfologi och därmed potentiella cytotoxicitet.

Tags

Biologi fråga 141 Low density lipoprotein Receptorn LDL tillströmning LDL upptag Dynasore Simvastatin rekombinant PCSK9 dynamin lipider kolesterol och åderförkalkning.
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