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Et fluorescens udsving spektroskopi Assay af Protein-Protein interaktioner på celle-celle kontakter
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Et fluorescens udsving spektroskopi Assay af Protein-Protein interaktioner på celle-celle kontakter

Article DOI: 10.3791/58582-v • 08:43 min • December 1st, 2018
December 1st, 2018 •
1, 1
1Institute for Biochemistry and Biology, Cell Membrane Biophysics Group, University of Potsdam

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Denne protokol beskriver et fluorescens udsving spektroskopi-baseret tilgang til at undersøge interaktioner mellem protein mægle celle-celle interaktioner, dvs protein lokaliseret i celle vejkryds, direkte i levende celler. Vi leverer detaljerede retningslinjer på instrument kalibrering, dataopsamling og analyse, herunder rettelser til mulige artefakt kilder.

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Biokemi sag 142 Protein-protein interaktioner celle-celle interaktioner celle-celle vedhæftning fluorescens udsving spektroskopi fluorescens-korrelations-spektroskopi antal og lysstyrke N & B
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