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Erkennung von Tilapia See Virus mit konventionellen RT-PCR und SYBR Green RT-qPCR
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Erkennung von Tilapia See Virus mit konventionellen RT-PCR und SYBR Green RT-qPCR

Article DOI: 10.3791/58596-v • 10:55 min • November 10th, 2018
November 10th, 2018 •
1, 2, 2
1Institute of Veterinary Bacteriology Vetsuisse Faculty, University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University

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Dieses Protokoll Diagnosen Tilapia See Virus (TiLV) in Tilapia Gewebe mit RT-PCR-Methoden. Die gesamte Methode ist von Gewebe Dissektion nach insgesamt RNA-Extraktion, gefolgt von cDNA Synthese und Erkennung von TiLV durch konventionelle PCR oder quantitative PCR mit DsDNA verbindlich einen Farbstoff Fluoreszenz Bindung beschrieben.

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Umweltwissenschaften Ausgabe 141 Tilapia See Virus Tilapia Diagnose RT-PCR RT-qPCR Orthomyxo-ähnliche Viren
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