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Upptäckt av Tilapia sjön Virus använder konventionella RT-PCR och SYBR Green RT-qPCR
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Upptäckt av Tilapia sjön Virus använder konventionella RT-PCR och SYBR Green RT-qPCR

Article DOI: 10.3791/58596-v • 10:55 min • November 10th, 2018
November 10th, 2018 •
1, 2, 2
1Institute of Veterinary Bacteriology Vetsuisse Faculty, University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University

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Detta protokoll diagnoser Tilapia sjön Virus (TiLV) i tilapia vävnader med hjälp av RT-PCR-metoder. Hela metoden beskrivs från vävnad dissektion till total RNA-extraktion, följt av cDNA syntes och upptäckt av TiLV av antingen konventionell PCR eller kvantitativ PCR med dsDNA bindande ett fluorescerande bindande färgämne.

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Fråga 141 tilapia Tilapia sjön virus miljövetenskap diagnos RT-PCR RT-qPCR orthomyxo-liknande virus
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