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JoVE Journal
Biology

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食品和环境样品中沙门氏菌的准总体基因体分析
 
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食品和环境样品中沙门氏菌的准总体基因体分析

Article DOI: 10.3791/58612-v 06:12 min October 25th, 2018
October 25th, 2018

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Summary

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在这里, 我们提出了一个协议, 准备从食物和环境微生物群 DNA 样本, 以协同检测和子类型沙门氏菌通过 quasimetagenomic 测序。结合使用培养富集、免疫磁分离 (IMS) 和多重置换扩增 (MDA), 可有效地将沙门氏菌基因组 DNA 从食物和环境样本中浓缩。

Tags

生物学 问题 140 quasimetagenomics 免疫磁分离 (IMS) 全基因组扩增 测序 检测 子类型,沙门氏菌
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